Article Details


Knockdown of C-C Chemokine Receptor 5 (CCR5) is Protective Against Cerebral Ischemia and Reperfusion Injury

[ Vol. 14 , Issue. 2 ]

Author(s):

Edna Constanza Gómez Victoria, Eliana Cristina de Brito Toscano, Ana Clara de Sousa Cardoso, Daniele Gonçalves da Silva, Aline Silva de Miranda, Lucíola da Silva Barcelos, Michelle Adriane Sugimoto, Lirlândia Pires Sousa, Isabel Vieira de Assis Lima, Antônio Carlos Pinheiro de Oliveira, Fátima Brant , Fabiana Simao Machado, Mauro Martins Teixeira, Antônio Lúcio Teixeira and Milene Alvarenga Rachid   Pages 125 - 131 ( 7 )

Abstract:


Background: Stroke is the second leading cause of death and a major cause of disability of adults worldwide. Inflammatory processes are known to contribute to the pathophysiology of cerebral ischemia, especially following reperfusion. Chemokines and their receptors are involved in migration of leukocytes and have been implicated in the pathogenesis of ischemic stroke.

Objective: In the present study, we investigated the effects of C-C chemokine receptor type 5 (CCR5) deficiency on neurological outcome, brain damage and expression of pro-inflammatory chemokines: chemokine (C-X-C motif) ligand 1 (CXCL1), chemokine (CC motif) ligand 3 (CCL3) and chemokine (C-C motif) ligand 5 (CCL5), and the brain-derived neurotrophic factor (BDNF).

Methods: Adult male C57BL/6 (wild-type) (WT) and CCR5 deficient mice were subjected to transient cerebral ischemia induced by 25 min of bilateral common carotid artery occlusion (BCCAO) followed by 24 hours of reperfusion. Mice were divided into four groups: WT sham group, which underwent sham operation; WT ischemic group, which was subjected to transient bilateral common carotid artery occlusion, CCR5-/- sham group, which underwent sham operation, and CCR5-/- ischemic group, which was subjected to transient BCCAO.

Results: In CCR5 deficiency, we observed a significant improvement in the neurological deficits associated with decreased brain infarcted area as evaluated by triphenyltetrazolium chloride (TTC). Moreover, CCR5 deficiency revealed decreased percentage of necrotic cavities areas and frequency of ischemic neurons by histometric analysis. In addition, CCR5-/- ischemic animals showed lower brain levels of the chemokine CXCL1 and higher levels of BDNF by ELISA, compared with WT BCCAo mice.

Conclusion: Taken together, our results suggest a potential neuroprotection in the absence of CCR5 receptor during global brain ischemia and reperfusion injury.

Keywords:

Brain, ischemia, reperfusion, chemokines, CCR5, BDNF, mice.

Affiliation:

Departamento de Patologia Geral, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Patologia Geral, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Patologia Geral, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Patologia Geral, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Fisiologia e Biofísica, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Farmacologia, Instituto de Ciências Biológicas (ICB), Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Farmacologia, Instituto de Ciências Biológicas (ICB), Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Bioquímica e Imunologia, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Bioquímica e Imunologia,Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Departamento de Bioquímica e Imunologia, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Neuropsychiatry Program, Department of Psychiatry and Behavioral Sciences, School of Medicine, University of Texas Health Science Center at Houston, TX, Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas. Departamento de Patologia Geral. Laboratorio de Apoptose. Campus Pampulha, Av. Antonio Carlos 6.627 - Belo Horizonte, Minas Gerais



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