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Potential contribution of microRNA-125b targeting p38MAPK to relieving intermittent hypoxia-induced dementia of mice models

Author(s):

Haiyan Ren, Weibin Qiu, Chen Peng, Ying Ding, Benju Zhu, Qiang Li and Xu Chen*  

Abstract:


Introduction: The purpose of this investigation was to elucidate whether miR-125b targeting p38MAPK could relieve cognitive impairment of obstructive sleep apnea hypopnea syndrome (OSAHS) mice models.

Material and methods: The mice models were categorized into untreated control (UC), 10% continuous hypoxic (CH), 10% chronic intermittent hypoxia (CIH) and 5% chronic intermittent hypoxia (CIH) groups. Morris Water Maze, spatial navigation test and spatial probe test were conducted to appraise the learning, memorizing and cognitive abilities of mice models. Moreover, miR-125b and p38MAPK expressions were figured out through performing quantitative real time-polymerase chain reaction (qRT-PCR) and western blotting. The luciferase reporter gene assay was also prepared to validate the targeted relationship between miR-125b and p38MAPK.

Results: Our study results showed that intermittent hypoxia generated more serious cognitive impairment of mice than persistent hypoxia (P < 0.05). Furthermore, miR-125b expression was down-regulated and phospho-p38MAPK expression was up-regulated significantly within intermittent hypoxia-treated mice (P < 0.05). Furthermore, the in-vitro tests exhibited that down-regulated expression of miR-125b and phosphorylation of p38MAPK could promote apoptosis and depress viability and proliferation of SH-SY5Y cells (P < 0.05).

Conclusion: miR-125b targeting p38MAPK could regulate cognition of OSAHS mice models by altering neuronal function of mice, which might provide theoretical basis for treating intermittent hypoxia-related dementia.

Keywords:

dementia, mouse model, miR-125b, p38MAPK, SH-SY5Y cells, intermittent hypoxia.

Affiliation:

Department of Neurology, Shanghai Eighth People’s Hospital, Shanghai, Department of Neurology, Shanghai Xuhui Central Hospital, Shanghai, Department of Neurology, Shanghai Eighth People’s Hospital, Shanghai, Department of Neurology, Shanghai Eighth People’s Hospital, Shanghai, Department of Neurology, Shanghai Eighth People’s Hospital, Shanghai, Department of Neurology, Shanghai Eighth People’s Hospital, Shanghai, Department of Neurology, Shanghai Eighth People’s Hospital, Shanghai



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