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MiR-125a-5p Alleviates Dysfunction and Inflammation of Pentylenetetrazol- induced Epilepsy Through Targeting Calmodulin-dependent Protein Kinase IV (CAMK4)

[ Vol. 16 , Issue. 4 ]

Author(s):

Qishuai Liu, Li Wang, Guizhen Yan, Weifa Zhang, Zhigang Huan and Jianyuan Li*   Pages 365 - 372 ( 8 )

Abstract:


Background: MicroRNAs (miRNA) are known to play a key role in the etiology and treatment of epilepsy through controlling the expression of gene. However, miR-125a-5p in the epilepsy is little known. Epilepsy in rat models was induced by Pentylenetetrazol (PTZ) and miR- 125a-5p profiles in the hippocampus were investigated in our experiment. Also, the relationship between miR-125a-5p and calmodulin-dependent protein kinase IV (CAMK4) was identified and the related mechanism was also illustrated.

Methods: The miR-125a-5p mRNA expression levels were evaluated by quantitative real time polymerase chain reaction (qRT-PCR). Western Blot (WB) was used to analyze the CAMK4 protein expression levels. Seizure score, latency and duration were determined based on a Racine scale. The enzyme-linked immunosorbent assay (ELISA) was used to analyze the inflammatory factor expression. The relationship between miR-125a-5p and CAMK4 was detected through dual luciferase assay.

Results: Downregulation of miR-125a-5p was observed in the hippocampus of PTZ-induced epilepsy rats. The overexpression of miR-125a-5p attenuated seizure and decreased inflammatory factor level in the hippocampus of PTZ-induced rats. The miR-125a-5p alleviated epileptic seizure and inflammation in PTZ-induced rats by suppressing its target gene, CAMK4.

Conclusion: miR-125a-5p may represent a novel therapeutic treatment for PTZ-induced epilepsy by preventing the activation of CAMK4.

Keywords:

Pentylenetetrazol, epilepsy, miR-125a-5p, CAMK4, dysfunction and inflammation, ELISA.

Affiliation:

Department of Neurology, The First Hospital of Zibo, Zibo City, Shandong Province, 255200, Department of Nephrology, The First Hospital of Zibo, Zibo City, Shandong Province, 255200, Department of Neurology, People's Hospital of Lixia District of Jinan, Jinan City, Shandong Province, 250014, Department of Neurology, The First Hospital of Zibo, Zibo City, Shandong Province, 255200, Department of Neurology, The First Hospital of Zibo, Zibo City, Shandong Province, 255200, Department of Neursurgery, Rizhao City Hospital of TCM, Rizhao City, Shandong Province, 276800



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