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MicroRNA (miR)-429 Promotes Inflammatory Injury by Targeting Kruppel-like Factor 4 (KLF4) in Neonatal Pneumonia

[ Vol. 17 , Issue. 1 ]

Author(s):

Lan Zhang*, HuanLi Yan, Huiping Wang, Li Wang, Boling Bai, Yingjun Ma, Yingchun Tie and Zhaoxia Xi   Pages 102 - 109 ( 8 )

Abstract:


Background: Neonatal pneumonia is a common disease in the neonatal period with a high incidence and death. This study aimed to investigate the molecular mechanism and effect of microRNA (miR)-429 in neonatal pneumonia.

Methods: The peripheral blood was collected from neonatal pneumonia and healthy patients, respectively. Human lung fibroblast WI-38 cells were treated with lipopolysaccharide (LPS) to establish neonatal pneumonia cell model. Then, the miR-429 expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR). In addition, the relationship between miR- 429 and kruppel-like factor 4 (KLF4) was confirmed by dual luciferase reporter assay. Cell viability, the level of interleukin 6 (IL-6), IL-1β and tumor necrosis factor α (TNF-α) and apoptosis were measured by Cell Counting Kit-8 (CCK-8), enzyme linked immunosorbent assay (ELISA) and flow cytometry. Meanwhile, apoptosis and nuclear factor kappa-B (NF-κB) pathway related proteins expression were analyzed by western blot.

Results: MiR-429 expression level was increased in neonatal peripheral blood and LPS-stimulated WI-38 cells. Then, miR-429 overexpression increased apoptosis, the level of IL-6, IL-1β, TNF-α, Bax and cleaved caspase-3, while reduced cell viability in LPS-stimulated WI-38 cells. Besides, KLF4 was identified as the target gene of miR-429, and reversed the changes caused by miR-429 overexpression. Finally, miR-429 suppressor down-regulated p-NF-κB level in LPS-stimulated cells and KLF4 knockdown reversed these reductions.

Conclusion: MiR-429 promotes inflammatory injury, apoptosis and activates the NF-κB signaling pathway by targeting KLF4 in neonatal pneumonia, and then these results provide evidence for clinical diagnosis and treatment for neonatal pneumonia.

Keywords:

Neonatal pneumonia, miR-429, kruppel-like factor 4 (KLF4), apoptosis, inflammatory injury, lipopolysaccharide (LPS).

Affiliation:

Department of Neonatology, Second Hospital of Xi'an Jiaotong University, Xi'an City Shaanxi Province, 710004, Department of Neonatology, The Second People's Hospital of Liaocheng, Liaocheng City, Shandong Province, 252600, Department of Neonatology, Second Hospital of Xi'an Jiaotong University, Xi'an City Shaanxi Province, 710004, Department of Neonatology, Second Hospital of Xi'an Jiaotong University, Xi'an City Shaanxi Province, 710004, Department of Neonatology, Second Hospital of Xi'an Jiaotong University, Xi'an City Shaanxi Province, 710004, Department of Neonatology, Second Hospital of Xi'an Jiaotong University, Xi'an City Shaanxi Province, 710004, Department of Neonatology, Second Hospital of Xi'an Jiaotong University, Xi'an City Shaanxi Province, 710004, Department of Neonatology, Second Hospital of Xi'an Jiaotong University, Xi'an City Shaanxi Province, 710004



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