Koichi Arimura, Tetsuro Ago, Masahiro Kamouchi, Kuniyuki Nakamura, Koji Ishitsuka, Junya Kuroda, Hiroshi Sugimori, Hiroaki Ooboshi, Tomio Sasaki and Takanari Kitazono Pages 1 - 9 ( 9 )
Platelet derived growth factor (PDGF)-B plays a neuroprotective role in brain damages, including ischemic stroke. It has been suggested recently that PDGF receptor β (PDGFRβ) expressed in brain pericytes as well as in neurons and astrocytes may mediate the neuroprotective role of PDGF-B. The aims of this study were to elucidate the roles of PDGFRβ signaling in brain pericytes after ischemic stroke. In a rat middle cerebral artery occlusion (MCAO) model, PDGFRβ expression was induced specifically in the pericytes in peri-infarct areas and its level was gradually increased. PDGF-B induced marked phosphorylation of Akt in cultured brain pericytes. Consistently, PDGF-B was upregulated in endothelial cells in per-infarct areas and Akt was strongly phosphorylated in the PDGFRβ-expressing pericytes in periinfarct areas after MCAO. In the cultured pericytes, PDGF-B induced cell growth and anti-apoptotic responses through Akt. Furthermore, PDGF-B significantly increased the expression of nerve growth factor (NGF) and neurotrophin-3 (NT- 3) through Akt in the pericytes. Thus, the PDGFRβ-Akt signaling in brain pericytes may play various important roles leading to neuroprotection after ischemic stroke.
angiogenesis, blood brain barrier, endothelial cells, microvascular, PI3K, Cell Signaling Technology, qPCR RT kit, LightCycler, SYBR, neurotrophic factor, Apoptosis, cell growth, ischemic stroke, neuroprotection, neurotrophins, pericyte, PDGF-B, PDGF receptor β
Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.