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CD31+ Circulating Angiogenic Cell Number and Subtypes are Reduced in Individuals with Chronic Stroke

[ Vol. 18 , Issue. 1 ]

Author(s):

Rian Q. Landers-Ramos*, Katherine I. Kim, Brent Hickey, Frederick M. Ivey, Charlene E. Hafer-Macko, Richard F. Macko, Alice S. Ryan and Steven J. Prior   Pages 113 - 122 ( 10 )

Abstract:


Background and Purpose: Reduced number and function of CD31+ circulating angiogenic cells (CACs) may explain vascular complications associated with the chronic phase stroke. The purpose of this study was to quantify CD31+ CAC paracrine function, total number and number of various subtypes of CD31+ CACs in individuals with chronic stroke compared with controls.

Methods: Peripheral blood mononuclear cells were isolated from chronic stroke participants and controls. CD31+ cells were quantified by flow cytometry, as was co-expression of CD31 in combination with CD14, CD3, CD11b, or CD34. Immunomagnetically selected CD31+ cells were cultured, and conditioned medium was used in a capillary-like network assay.

Results: Significantly lower levels of CD31+ CACs were found in stroke participants compared with controls (-24%; P=0.04). Additionally, CD31+/CD14+, CD31+/CD11b+ and CD31+/CD3+ cells were significantly lower in the chronic stroke group compared with controls (-45%, P=0.02; -47%, P=0.02 and -32%, P=0.03, respectively). There was no group effect on CD31+ CAC conditioned media-mediated capillary-like network formation.

Conclusion: CD31+ CACs and subtypes may serve as potential therapeutic targets in chronic stroke recovery.

Keywords:

Circulating angiogenic cells, CD31, chronic stroke, angiogenic T cell, paracrine, angiogenesis.

Affiliation:

Department of Kinesiology, Towson University, Towson, MD, Department of Kinesiology, University of Maryland School of Public Health, College Park, MD, Department of Kinesiology, Towson University, Towson, MD, Geriatric Research Education and Clinical Center, Baltimore Veterans Affairs Medical Center, Baltimore, MD, Geriatric Research Education and Clinical Center, Baltimore Veterans Affairs Medical Center, Baltimore, MD, Geriatric Research Education and Clinical Center, Baltimore Veterans Affairs Medical Center, Baltimore, MD, Geriatric Research Education and Clinical Center, Baltimore Veterans Affairs Medical Center, Baltimore, MD, Geriatric Research Education and Clinical Center, Baltimore Veterans Affairs Medical Center, Baltimore, MD



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